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Oni2:Slaves of War/Polylectiloquy: Difference between revisions
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:Kerr tells us that the Chrysalises were being grown "based on the genetic codes of" Mai and Muro. One way to interpret that is that they had extracted portions of tissue, or perhaps used their stem cells to generate a new mass of tissue, and introduced the Daodan to them; perhaps they used a sample from a plant or animal in the Wilderness that they found to be Daodan-enhanced. If the Daodan organism is something as low-level as a genetic process (see the [[Oni2:Slaves of War/Neo-Biology#Daodan organism|Neo-Biology]] page for details), then the idea is that it can leap from species to species, so the source of the Daodan "genes" is not important. Then, when it was eventually decided to implant the Daodanized tissue back into Mai and Muro, Hasegawa and Kerr would have needed some way to take the tissue that had been modified outside their bodies and re-introduce it. | :Kerr tells us that the Chrysalises were being grown "based on the genetic codes of" Mai and Muro. One way to interpret that is that they had extracted portions of tissue, or perhaps used their stem cells to generate a new mass of tissue, and introduced the Daodan to them; perhaps they used a sample from a plant or animal in the Wilderness that they found to be Daodan-enhanced. If the Daodan organism is something as low-level as a genetic process (see the [[Oni2:Slaves of War/Neo-Biology#Daodan organism|Neo-Biology]] page for details), then the idea is that it can leap from species to species, so the source of the Daodan "genes" is not important. Then, when it was eventually decided to implant the Daodanized tissue back into Mai and Muro, Hasegawa and Kerr would have needed some way to take the tissue that had been modified outside their bodies and re-introduce it. | ||
:The means for this process can be found in a cutting-edge field known as [[wikipedia:Regenerative_medicine|regenerative medicine]]. Papers such as [ | :The means for this process can be found in a cutting-edge field known as [[wikipedia:Regenerative_medicine|regenerative medicine]]. Papers such as [https://www.freshpatents.com/Multilayered-composite-for-organ-augmentation-and-repair-dt20080403ptan20080081362.php this patent application] <!--alternate link from USPTO itself, not sure if it expires after a while: http://appft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PG01&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.html&r=1&f=G&l=50&s1=%2220080081362%22.PGNR.&OS=DN/20080081362&RS=DN/20080081362--> demonstrate a means by which autologous cells (one's own cells, not a donor's) can be extracted, altered, and re-implanted using a degradable matrix. As ¶30 says, | ||
{{Pullquote|Alternatively, the tissue or cellularized (cell) sample may be treated in vitro before being placed on the scaffold layer. For example, cells (such as autologous cells) can be cultured in vitro to increase the number of cells available for seeding on the scaffold(s). The use of allogenic cells, and more preferably autologous cells, is preferred to prevent tissue rejection. In certain embodiments, chimeric cells, or cells from a transgenic animal, can be seeded onto the polymeric matrix. Cells can also be transfected prior to seeding with genetic material. Useful genetic material may be, for example, genetic sequences which are capable of reducing or eliminating an immune response in the host. For example, the expression of cell surface antigens such as class I and class II histocompatibility antigens may be suppressed. This may allow the transplanted cells to have reduced chance of rejection by the host. In addition, transfection could also be used for gene delivery. Urothelial and muscle cells could be transfected with specific genes prior to polymer seeding. The cell-polymer construct could carry genetic information required for the long term survival of the host or the tissue engineered neo-organ.}} | {{Pullquote|Alternatively, the tissue or cellularized (cell) sample may be treated in vitro before being placed on the scaffold layer. For example, cells (such as autologous cells) can be cultured in vitro to increase the number of cells available for seeding on the scaffold(s). The use of allogenic cells, and more preferably autologous cells, is preferred to prevent tissue rejection. In certain embodiments, chimeric cells, or cells from a transgenic animal, can be seeded onto the polymeric matrix. Cells can also be transfected prior to seeding with genetic material. Useful genetic material may be, for example, genetic sequences which are capable of reducing or eliminating an immune response in the host. For example, the expression of cell surface antigens such as class I and class II histocompatibility antigens may be suppressed. This may allow the transplanted cells to have reduced chance of rejection by the host. In addition, transfection could also be used for gene delivery. Urothelial and muscle cells could be transfected with specific genes prior to polymer seeding. The cell-polymer construct could carry genetic information required for the long term survival of the host or the tissue engineered neo-organ.}} | ||
:This method can work not just for replacing missing organs, but, as ¶31 says, | :This method can work not just for replacing missing organs, but, as ¶31 says, | ||